Abstract. The pronounced neurotoxicity of the potent antitumor drug cisplatin frequently results in the onset of peripheral polyneuropathy (PNP), which is assumed to be initially triggered by platination products in the nuclear DNA of affected tissues. To further elucidate the molecular mechanisms, we analyzed in a mouse model the formation and processing of the main cisplatin-induced DNA adduct (guanine-guanine intrastrand cross-link) in distinct neuronal cell types by adduct-specific

Cisplatin interagerar också med DNA, inducerande monoadducts, intrastrand cross-links och ICLs mellan guaninrester; I motsats till MMC krävs emellertid inte

To further elucidate the molecular mechanisms, we analyzed in a mouse model the formation and processing of the main cisplatin-induced DNA adduct (guanine-guanine intrastrand cross-link) in distinct neuronal cell types by adduct-specific Transplatin, (an isomer of cisplatin that has the chlorine atoms opposite each other, rather than on the same side) also forms adducts with DNA, but mostly mono-adducts. It does not promote cross-linking which is the cause of the gene replication process. The cisplatin molecule binds with a protein on one side and the DNA molecule on the other. For nuclear genomes, cisplatin–DNA adducts are enriched within promoters and regions harboring transcription termination sites. While the density of GG dinucleotides determines the initial crosslinking of cisplatin, binding of proteins to the genome largely contributes to the accumulative pattern of cisplatin–DNA adducts. 1.

Bosutinib Exerts anti-tumor activity by producing DNA adducts that can The platinum (Pt) drugs cisplatin and carboplatin are heavily employed in chemotherapy Structure, Recognition, and Processing of Cisplatin-DNA Adducts. Kemikalier som bildar DNA-addukter inkluderar: acetaldehyd , en betydande beståndsdel av tobaksrök; cisplatin , som binder till DNA och orsakar tvärbindning, Anti-Cisplatin DNA Adducts, clone ICR4 (rat monoclonal). Produktnummer. : MABE416. Artikelnummer.

An immunohistochemical assay for analysis of CDDP-DNA adducts, i.e. the drug in its probable target position, was modified to allow quantitation with

1994;(125):339-48. DNA adducts of cisplatin, transplatin and platinum-intercalating drugs. Leng M(1), Brabec V. Author information: (1)Centre de Biophysique Moléculaire, Centre Nationale de la Recherche Scientifique, Orléans, France. Cisplatin is a major cancer chemotherapeutic drug.

1 Jan 2006 Effects of gemcitabine on cis-platinum-DNA adduct formation and repair in a panel of gemcitabine and cisplatin-sensitive or -resistant human

Previously, it was reported that Covering the both BRCA genes to screen for mutations in the complete coding region including +50 bp up- and downstream of each coding exon of the BRCA1 An immunohistochemical assay for analysis of CDDP-DNA adducts, i.e. the drug in its probable target position, was modified to allow quantitation with Visar resultat 1 - 5 av 49 avhandlingar innehållade orden DNA adducts. Studies of Chemotherapeutic Drug Cisplatin : Activation and Binding to DNA. Swedish University dissertations (essays) about DNA ADDUCTS. Search and Pharmacokinetic and pharmacodynamic studies on cisplatin in mice and men. However, the cellular accumulation, efflux, steady state content, and the formation of DNA adducts of the cisplatin [H-3]-DEP were not affected by adenosine- av O Hemmingsson · 2010 — Damage recognition protein HMG1 shields DNA from repair and is overexpressed in resistant cells (36). Apoptosis. Once cisplatin-DNA adducts are recognized av B Andersson · 2007 — Investigate if cisplatin-induced apoptosis in P31 wt and P31 res cells could be "Effects of cisplatin and amphotericin B on DNA adduct formation and toxicity in.

Studies of Chemotherapeutic Drug Cisplatin : Activation and Binding to DNA. Swedish University dissertations (essays) about DNA ADDUCTS. Search and Pharmacokinetic and pharmacodynamic studies on cisplatin in mice and men. However, the cellular accumulation, efflux, steady state content, and the formation of DNA adducts of the cisplatin [H-3]-DEP were not affected by adenosine- av O Hemmingsson · 2010 — Damage recognition protein HMG1 shields DNA from repair and is overexpressed in resistant cells (36). Apoptosis. Once cisplatin-DNA adducts are recognized av B Andersson · 2007 — Investigate if cisplatin-induced apoptosis in P31 wt and P31 res cells could be "Effects of cisplatin and amphotericin B on DNA adduct formation and toxicity in. A Trial of Gemcitabine, Infusional 5-Fluorouracil and Cisplatin for Advanced Platinum compounds lead to cell death by forming DNA adducts and causing av CL Loprinzi · Citerat av 7 — Paclitaxel and Cisplatin-induced neurotoxicity: a protective role of effect on oxaliplatin pharmacokinetics, Pt-DNA adduct formation, and DNA repair and replication influence the number of mutations per adduct of that mitomycin C or cisplatin-induced DNA lesions are not incised by global… these authorities discuss the chemistry of cisplatin in aqueous solution, the DNA and how recognitions and processing of platinum-DNA adducts may be of cisplatin-induced ototoxicity include the formation of.
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Cisplatin-DNA adducts are molecular decoys for the ribosomal RNA transcription factor hUBF (human upstream binding factor) D K Treiber, X Zhai, H M Jantzen, and J M Essigmann. Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139. See all. Hide.

I. Gill,1 F. M. Key words: cisplatin, carboplatin, platinum-DNA adduct, phase I. In 23.8 mM carbonate buffer, 5 mM NaCl, pH 7.4, cisplatin forms carbonato species that produce DNA-adducts which do not significantly change supercoiling but Abstract. The antitumor agent cis -diamminedichloroplatinum(II) (cisplatin) introduces cytotoxic DNA damage predominantly in the form of intrastrand crosslinks.
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17 Apr 2013 Conclusion Our findings suggest that the cisplatin-DNA adduct level is the most important determinant of cisplatin sensitivity in HNSCC cells.

For nuclear genomes, cisplatin–DNA adducts are enriched within promoters and regions harboring transcription termination sites. While the density of GG dinucleotides determines the initial crosslinking of cisplatin, binding of proteins to the genome largely contributes to the accumulative pattern of cisplatin–DNA adducts.

Kemikalier som bildar DNA-addukter inkluderar: acetaldehyd , en betydande beståndsdel av tobaksrök; cisplatin , som binder till DNA och orsakar tvärbindning,

av M Johansson — behovet att genomföra analyser av cirkulerande DNA. Detta kommer att Standardbehandlingen är cisplatin i kombination med vinorelbin.

Material precursors in the form of Cisplatin adducts are Guanine-Guanine (GG) DNA intrastrand cross links caused by the chemical compound Cisplatin. Cisplatin is used as a chemotherapy agent 1 Jan 2006 Effects of gemcitabine on cis-platinum-DNA adduct formation and repair in a panel of gemcitabine and cisplatin-sensitive or -resistant human 13 Oct 2016 Abstract Cisplatin, one of the most widely used anticancer drugs, crosslinks DNA and ultimately induces cell death. However, the genomic 17 Apr 2013 Conclusion Our findings suggest that the cisplatin-DNA adduct level is the most important determinant of cisplatin sensitivity in HNSCC cells. accumulation were prominent features of the cisplatin-DNA adduct profile. Functional DNA repair capacity has been studied in eight human leukocyte cell lines 18 Jan 2019 Cisplatin interacts with DNA mainly in the form of Pt-d(GpG) di-adduct, which stalls cell proliferation and activates DNA damage response.